Join us
Are you an undergrad or graduate student at JHU looking for a research project in stem cell and developmental biology, multi-omics bioinformatic analysis, or translational applications of human pluripotent stem cells including retinal, vascular, hematopoietic regeneration and immune cell therapy? Are you a local or international postdoctoral fellow who wants to develop a research project in regenerative medicine together? We regularly employ up to 10 master and undergrad students from various backgrounds such as Biotechnology, Biomedical Engineering, Premed and multiple MD or PhD postdoctoral fellows at the Zambidis lab. The Zambidis lab is a diverse space with members from all over the globe.
Depending on your previous experience and the specifics of your project, we’ll train you in basic and advanced laboratory techniques incl. flow cytometry, RNA / DNA isolation and cloning, RT-PCR, western blotting, maintaining, transfecting, and differentiating stem cell lines under various conditions incl. our patented blastomere-like TIRN stem cell technology (Park et al. 2016, Zimmerlin et al., 2025), mouse colony management and biomethodologies up to tail-vein injections and simple survival surgeries. We regularly differentiate human ESC, iPSC, and TIRN stem cells in vitro and in vivo towards various embryonic and somatic lineages of all three germ layers incl. Trophectoderm, retinal organoids and progenitors, vascular progenitors, and hematopoietic lineages with a focus on embryonic and fetal myeloid hematopoiesis. The Zambidis lab has generated a plethora of iPSC lines from adult and neonatal blood monocytes and endothelial cells, mesenchymal stem cells, and fibroblasts, from healthy and disease backgrounds, many of which are commercially available. We have over a decade of experience with cutting edge Omics techniques and analyses, incl. bulk and single cell RNA seq, mass spec proteomics, ubiquitinomics, methylomics, and chromatin accessibility.
You’ll have the chance to present your research at internal, national, and international conferences and network with fellow researchers and I’ll support you to write your own research proposals, grant and program applications. We also regularly hire students as lab technicians or coordinators and involve you in all important lab processes, as well as providing guidance to develop your own project within the Zambidis lab.
Interested? Contact us with a biosketch detailing your scholastic and research background to apply for an available project or to develop a project together.
Current Projects:
- Epigenetic characterization and developmental potential of pre-naive induced totipotent-like TIRN stem cells: Integrated embryo models and blastocyst complementation towards ethically sound lineage-restricted interspecies chimeras for the generation of cell therapies and transplant tissues.
Generation of a HLA-typed xeno-/ feeder-/ transgene-free cGMP-ready human pluripotent stem cell bank from various healthy and disease backgrounds both as iPSC and ESC. - Neuroretinal differentiation of TIRN compared to conventional iPSC: Molecular identity and in vivo integration.
- Stem Cell-based myeloid hematopoiesis for clinical applications in oncology (e.g., chimeric antigen receptor macrophages), hematology and infectious diseases (e.g., antibiotic-resistant neutropenic fever, fungal and other hard-to-treat infections), and immunology / rheumatology (auto-/ allo-tolerance induction).
